triage_status large_stringclasses 28
values | pmid large_stringlengths 7 13 | abstract large_stringlengths 49 3.69k | citation large_stringlengths 16 84 | token_count int32 17 878 | label int8 0 1 |
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Curatable | PMID:19804756 | The Mre11/Rad50/Nbs1 protein complex plays central enzymatic and signaling roles in the DNA-damage response. Nuclease (Mre11) and scaffolding (Rad50) components of MRN have been extensively characterized, but the molecular basis of Nbs1 function has remained elusive. Here, we present a 2.3A crystal structure of the N-t... | Cell 2009 Oct 02;139(1):100-11 | 266 | 1 |
Phylogeny and evolutionary studies | PMID:17138626 | The DJ-1 gene is extensively studied because of its involvement in familial Parkinson disease. DJ-1 belongs to a complex superfamily of genes that includes both prokaryotic and eukaryotic representatives. We determine that many prokaryotic groups, such as proteobacteria, cyanobacteria, spirochaetes, firmicutes, or fuso... | Mol Biol Evol 2007 Feb;24(2):551-61 | 283 | 0 |
Method or reagent | PMID:30108134 | Identifying critical pathways governing disease progression is essential for accurate prognosis and effective therapy. We developed a broadly applicable and novel systems-level gene discovery strategy. This approach focused on constitutively active androgen receptor (AR) splice variant-driven pathways as representative... | Mol Syst Biol 2018 08 14;14(8):e8202 | 237 | 0 |
Curatable | PMID:25330182 | Php4 is a nucleo-cytoplasmic shuttling protein that accumulates in the nucleus during iron deficiency. When present in the nucleus, Php4 associates with the CCAAT-binding protein complex and represses genes encoding iron-using proteins. Here, we show that nuclear import of Php4 is independent of the other subunits of t... | PLoS One 2014;9(10):e110721 | 305 | 1 |
Curatable | PMID:20959444 | In fission yeast, the endoplasmic reticulum membrane-bound proteins Sre1 and Scp1, orthologs of mammalian sterol regulatory element binding protein (SREBP) and Scap, monitor sterol synthesis as an indirect measure of oxygen supply. When cellular oxygen levels are low, sterol synthesis is inhibited, and the Sre1-Scp1 co... | J Biol Chem 2010 Dec 24;285(52):41051-61 | 237 | 1 |
Curatable | PMID:32101481 | In many organisms, positive and negative signals cooperate to position the division site for cytokinesis. In the rod-shaped fission yeast Schizosaccharomyces pombe , symmetric division is achieved through anillin/Mid1-dependent positive cues released from the central nucleus and negative signals from the DYRK-family p... | Mol Biol Cell 2020 04 15;31(9):917-929 | 239 | 1 |
Wrong organism | PMID:23359466 | Regulation of actomyosin ring contraction is important for the coordination of cytokinesis with mitosis. Hof1, a member of the Pombe Cdc15 homology (PCH) family of proteins, is required for efficient cytokinesis in budding yeast. Phosphorylation of Hof1 depends on the mitotic exit network (MEN), and its degradation at ... | Cell Biol Int 2013 Apr;37(4):314-25 | 221 | 0 |
Curatable | PMID:19686603 | DNA polymerase delta plays an essential role in chromosomal DNA replication in eukaryotic cells, being responsible for synthesising the bulk of the lagging strand. In fission yeast, Pol delta is a heterotetrameric enzyme comprising four evolutionarily well-conserved proteins: the catalytic subunit Pol3 and three smalle... | BMC Mol Biol 2009 Aug 17;10:82 | 382 | 1 |
Wrong organism | PMID:9655500 | Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. One of the septins,... | Neuron 1998 Jun;20(6):1111-22 | 192 | 0 |
Cell composition or WT feature | PMID:12109879 | Twenty-seven Schizosaccharomyces pombe isolates from seven cachaça distilleries were tested for maximum temperature of growth and fermentation, osmotolerance, ethanol resistance, invertase production, and trehalose accumulation. Two isolates were selected for studies of trehalose accumulation under heat shock and ethan... | Can J Microbiol 2002 May;48(5):399-406 | 336 | 0 |
Wrong organism | PMID:25847193 | Cyclophilin 18-2 (CYP18-2) genes, homologues of human peptidyl-prolyl isomerase-like 1 (PPiL1), are conserved across multicellular organisms and Schizosaccharomyces pombe. Although PPiL1 is known to interact with ski-interacting protein (SKIP), a transcriptional co-regulator and spliceosomal component, there have been ... | Plant Cell Environ 2015 Oct;38(10):2071-87 | 407 | 0 |
Curatable | PMID:25720772 | Complex phosphorylation-dependent signaling networks underlie the coordination of cellular growth and division. In the fission yeast Schizosaccharomyces pombe, the Dual specificity tyrosine-(Y)-phosphorylation regulated kinase (DYRK) family protein kinase Pom1 regulates cell cycle progression through the mitotic induce... | Mol Cell Proteomics 2015 May;14(5):1275-87 | 308 | 1 |
Curatable | PMID:15485922 | It has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-r... | Mol Cell Biol 2004 Nov;24(21):9557-67 | 318 | 1 |
Wrong organism | PMID:29164392 | Activated dendritic cells (DC) induce and polarize T-cell responses by expression of distinct maturation markers and cytokines. This study systematically investigated the capacity of different biotechnically relevant yeast species and strains including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces ... | Med Microbiol Immunol 2018 Feb;207(1):75-81 | 395 | 0 |
Curatable | PMID:22425159 | Vigorous chromosome movements driven by cytoskeletal assemblies are a widely conserved feature of sexual differentiation to facilitate meiotic recombination. In fission yeast, this process involves the dramatic conversion of arrays of cytoplasmic microtubules (MTs), generated from multiple MT organizing centers (MTOCs)... | Curr Biol 2012 Apr 10;22(7):562-74 | 340 | 1 |
Wrong organism | PMID:21205177 | Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. He... | J Integr Plant Biol 2011 Jan;53(1):14-24 | 287 | 0 |
Review or comment | PMID:22561213 | The centromere is the fundamental unit for insuring chromosome inheritance. This complex region has a distinct type of chromatin in which histone H3 is replaced by a structurally different homologue identified in humans as CENP-A. In metazoans, specific DNA sequences are neither required nor sufficient for centromere i... | Exp Cell Res 2012 Jul 15;318(12):1353-60 | 244 | 0 |
Curatable | PMID:24787148 | AMSH, a conserved zinc metallo deubiquitinase, controls downregulation and degradation of cell-surface receptors mediated by the endosomal sorting complexes required for transport (ESCRT) machinery. It displays high specificity toward the Lys63-linked polyubiquitin chain, which is used as a signal for ESCRT-mediated en... | Biochemistry 2014 May 20;53(19):3199-217 | 614 | 1 |
Wrong organism | PMID:2648130 | The mouse Surfeit locus, which contains a cluster of at least four genes (Surf-1 to Surf-4), is unusual in that adjacent genes are separated by no more than 73 base pairs (bp). The heterogeneous 5' ends of Surf-1 and Surf-2 are separated by only 15 to 73 bp, the 3' ends of Surf-1 and Surf-3 are only 70 bp apart, and th... | Mol Cell Biol 1989 Jan;9(1):224-31 | 445 | 0 |
Modelling | PMID:32692956 | Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species. In fission yeast Schizosaccharomyces pombe , DNA of high AT content is a distinct feature of replicatio... | Open Biol 2020 07;10(7):200052 | 279 | 0 |
Modelling | PMID:22094428 | Mother cell-specific ageing is a well-known phenomenon in budding yeast Saccharomyces cerevisiae. Asymmetric segregation of damage and its accumulation in the mother cell has been proposed as one important mechanism. There are, however, unicellular organisms such as the fission yeast Schizosaccharomyces pombe, which re... | Subcell Biochem 2012;57:315-30 | 264 | 0 |
Method or reagent | PMID:20617397 | There is a rapidly growing demand for fluorescent single-chain Fv (scFv) antibody fragments for many applications. Yeasts have developed into attractive hosts for recombinant production of these functionalized proteins because they provide several advantages over prokaryotes and higher eukaryotes as expression systems,... | Appl Biochem Biotechnol 2011 Jan;163(1):80-9 | 312 | 0 |
Review or comment | PMID:28929213 | Isoprenylcysteine-O-Carboxyl Methyltransferase (ICMT) catalyzes the final step in the prenylation process of different proteins including members of the Ras superfamily of GTPases. While cysteine methylation is essential in mammalian cells for growth, membrane association, and signalling by Ras and Rho GTPases, its rol... | Curr Genet 2018 Apr;64(2):341-344 | 235 | 0 |
Curatable | PMID:20601686 | Removal of the conserved telomere protein, Pot1, confers the immediate loss of fission yeast telomeres. This drastic phenotype has established the centrality of Pot1 for telomere maintenance but prohibited elucidation of the intermediate steps leading to telomere loss. To circumvent this problem, we have generated a co... | Nucleic Acids Res 2010 Nov;38(20):6968-75 | 156 | 1 |
Method or reagent | PMID:1502179 | Resolution of whole cell extract through two chromatographic steps yields a single protein fraction requiring only the addition of TFIID for the initiation of transcription at RNA polymerase II promoters. This approach allows the convenient generation of RNA polymerase II transcription systems from Saccharomyces cerevi... | Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7659-63 | 162 | 0 |
Curatable | PMID:17456468 | The DNA damage checkpoint pathway governs how cells regulate cell cycle progression in response to DNA damage. A screen for suppressors of a fission yeast chk1 mutant defective in the checkpoint pathway identified a novel Schizosaccharomyces pombe protein, Msc1. Msc1 contains 3 plant homeodomain (PHD) finger motifs, ch... | J Biol Chem 2007 Jun 22;282(25):18397-18406 | 369 | 1 |
Curatable | PMID:28357272 | Yeasts provide an excellent genetically tractable eukaryotic system for investigating the function of genes in their biological context, and are especially relevant for those conserved genes that cause disease. We study the role of btn1 , the orthologue of a human gene that underlies an early onset neurodegenerative d... | Microb Cell 2015 Nov 11;2(12):466-480 | 241 | 1 |
Wrong organism | PMID:18355438 | Dolichol phosphate mannose synthase (DPM) catalyzes the reaction between dolichol phosphate (Dol-P) and guanosine diphosphate mannose (GDP-Man) to form dolichol-phosphate-mannose (Dol-P-Man). This molecule acts as mannose donor for N-glycosylation and glycosylphosphatidylinositol (GPI) biosynthesis. The Plasmodium falc... | Biochem Biophys Res Commun 2008 Jun 06;370(3):388-93 | 291 | 0 |
Other | PMID:3233558 | The growth patterns of individual cells of the fission yeast (Schizosaccharomyces pombe wild-type cells, strain 972 h-; cells exposed to hydroxyurea; and cdc mutants, 11-123, 2-33) were investigated by time-lapse photomicrography. Wild-type cells showed one, two, or three linear-growth segments followed by a constant-l... | Can J Microbiol 1988 Dec;34(12):1338-43 | 274 | 0 |
Curatable | PMID:30089114 | Centromere is a specialized chromatin domain that plays a vital role in chromosome segregation. In most eukaryotes, centromere is surrounded by the epigenetically distinct heterochromatin domain. Heterochromatin has been shown to contribute to centromere function, but the precise role of heterochromatin in centromere s... | PLoS Genet 2018 08;14(8):e1007572 | 401 | 1 |
Curatable | PMID:10547374 | We recently identified Msp1p, a fission yeast Schizosaccharomyces pombe dynamin-related protein, which is essential for the maintenance of mitochondrial DNA. The Msp1p sequence displays typical features of a mitochondrial protein. Here we report in vitro and in vivo data that validate that prediction. We demonstrate th... | J Cell Sci 1999 Nov;112 ( Pt 22):4151-61 | 252 | 1 |
Loaded in error | PMID:25174444 | We present a novel parameter identification algorithm for the estimation of parameters in models of cell motility using imaging data of migrating cells. Two alternative formulations of the objective functional that measures the difference between the computed and observed data are proposed and the parameter identificat... | J Math Biol 2015 Aug;71(2):399-436 | 204 | 0 |
Curatable | PMID:16842820 | The protection of telomeres 1 (Pot1) proteins specifically recognize the single-stranded 3' end of the telomere, an activity essential for sustained cellular viability and proliferation. The current model for the telomeric single-stranded DNA (ssDNA) binding activity of Schizosaccharomyces pombe Pot1 is based on a 20 k... | J Mol Biol 2006 Aug 04;361(1):80-93 | 431 | 1 |
Curatable | PMID:12893961 | Saccharomyces cerevisiae A49 and mouse PAF53 are subunits specific to RNA polymerase I (Pol I) in eukaryotes. It has been known that Pol I without A49 or PAF53 maintains non-specific transcription activities but a molecular role(s) of A49 (and PAF53) remains totally unknown. We studied the fission yeast gene encoding a... | Genes Genet Syst 2003 Jun;78(3):199-209 | 305 | 1 |
Review or comment | PMID:24256273 | Sexual reproduction is a fundamental aspect of eukaryotic cells, and a conserved feature of gametogenesis is its dependency on a master regulator. The ste11 gene was isolated more than 20 years ago by the Yamamoto laboratory as a suppressor of the uncontrolled meiosis driven by a pat1 mutant. Numerous studies from this... | Biochem Soc Trans 2013 Dec;41(6):1673-8 | 232 | 0 |
Review or comment | PMID:35325114 | Leucine (Leu) is a branched-chain, essential amino acid in animals, including humans. Fungi, including the fission yeast Schizosaccharomyces pombe, can biosynthesize Leu, but deletion of any of the genes in this biosynthesis leads to Leu auxotrophy. In this yeast, although a mutation in the Leu biosynthetic pathway, le... | FEMS Yeast Res 2022 Mar 24; | 299 | 0 |
Wrong organism | PMID:12056916 | p34, a specific p-nitrophenyl phosphatase (pNPPase) was identified and purified from the murine cell line EL4 in a screen for the intracellular molecular targets of the antiinflammatory natural product parthenolide. A BLAST search analysis revealed that it has a high degree of sequence similarity to two yeast alkaline ... | Biochemistry 2002 Jun 18;41(24):7841-8 | 315 | 0 |
Wrong organism | PMID:14519125 | Mammalian translation initiation factor 3 (eIF3) is a multisubunit complex containing at least 12 subunits with an apparent aggregate mass of approximately 700 kDa. eIF3 binds to the 40S ribosomal subunit, promotes the binding of methionyl-tRNAi and mRNA, and interacts with several other initiation factors to form the ... | Eur J Biochem 2003 Oct;270(20):4133-9 | 429 | 0 |
Wrong organism | PMID:9884240 | Fission yeast cells grow by extension at the ends (poles) and divide by transverse fission. It has previously been reported that Schizosaccharomyces japonicus var. japonicus can switch to unipolar, filamentous growth. Here it is shown that the yeast-to-mycelium transition is a gradual process involving a changeover to ... | Microbiology (Reading) 1998 Dec;144 ( Pt 12):3475-3485 | 208 | 0 |
Curatable | PMID:10701132 | In the fission yeast, four genes (rpaP1-1, rpaP1-3, rpaP2-2, and rpaP2-4) encoding two variants of the RpaP1 and RpaP2 ribosomal proteins (rp) have been characterized. We have identified cDNA for additional variants called RpaP1.5 and RpaP2.6. Sequence comparison suggests that RpaP1.5 diverged before RpaP1.1 and RpaP1.... | Genome 2000 Feb;43(1):205-7 | 170 | 1 |
Wrong organism | PMID:23583778 | The anaphase-promoting complex or cyclosome (APC/C) is a large E3 RING-cullin ubiquitin ligase composed of between 14 and 15 individual proteins. A striking feature of the APC/C is that only four proteins are involved in directly recognizing target proteins and catalyzing the assembly of a polyubiquitin chain. All othe... | J Mol Biol 2013 Nov 15;425(22):4236-48 | 446 | 0 |
Curatable, low priority | PMID:2880751 | Purified soluble H+-ATPase from Schizosaccharomyces pombe catalyzes a Pi in equilibrium ATP exchange in the absence of a H+ gradient. When the pH of the assay medium is raised from 5.5 to 8.0 there is a decrease of the ATPase activity and an increase of the rate of Pi in equilibrium ATP exchange. At pH 7.0 the addition... | FEBS Lett 1987 Feb 23;212(2):323-7 | 145 | 1 |
Wrong organism | PMID:24185989 | Protoplasts of petites of strains 625-C(I) of Saccharomyces diastaticus and NCYC 1085 of Saccharomyces cerevisiae, originally obtained from the National Collection of Yeast Cultures, England, were fused with protoplasts of Candida pseudotropicalis, Saccharomyces rosei, Yaccharbmycesmontanus, Pichiamembranefaciens, Hans... | Curr Genet 1981 Dec;4(3):177-80 | 464 | 0 |
Curatable | PMID:15800064 | Microtubules regulate diverse cellular processes, including chromosome segregation, nuclear positioning, and cytokinesis. In many organisms, microtubule nucleation requires gamma-tubulin and associated proteins present at specific microtubule organizing centers (MTOCs). In fission yeast, interphase cytoplasmic microtub... | Mol Biol Cell 2005 Jun;16(6):3052-63 | 336 | 1 |
Review or comment | PMID:15053869 | The pairing of homologous chromosomes is a universal feature of meiosis and is important for the accurate segregation of chromosomes in the first of two meiotic divisions. In the March issue of Developmental Cell, report findings in fission yeast which point to telomere clustering and movement as being important determ... | Mol Cell 2004 Mar 26;13(6):766-8 | 69 | 0 |
Wrong organism | PMID:10676638 | Many cancer therapies cause DNA damage to effectively kill proliferating tumor cells; however, a major limitation of current therapies is the emergence of resistant tumors following initial treatment. Cell cycle checkpoints are involved in the response to DNA damage and specifically prevent cell cycle progression to al... | Cancer Res 2000 Feb 01;60(3):566-72 | 380 | 0 |
Curatable | PMID:28539404 | We used quantitative confocal microscopy and FPALM superresolution microscopy of live fission yeast to investigate the structures and assembly of two types of interphase nodes-multiprotein complexes associated with the plasma membrane that merge together and mature into the precursors of the cytokinetic contractile rin... | Mol Biol Cell 2017 Nov 07;28(23):3203-3214 | 272 | 1 |
Curatable | PMID:7706287 | A collection of fission yeast Schizosaccharomyces pombe conditional mutants was screened for defective nucleocytoplasmic transport of poly(A)+ RNA by fluorescence in situ hybridization. We identified a temperature-sensitive mutant that accumulated poly(A)+ RNA in the nucleus and have named it rae1-1, for ribonucleic ac... | J Biol Chem 1995 Mar 31;270(13):7411-9 | 249 | 1 |
Other | PMID:17512413 | Chromosomal regions can adopt stable and heritable alternative states resulting in bistable gene expression without changes to the DNA sequence. Such epigenetic control is often associated with alternative covalent modifications of histones. The stability and heritability of the states are thought to involve positive f... | Cell 2007 May 18;129(4):813-22 | 208 | 0 |
Wrong organism | PMID:2142304 | The a mating-type region of Neurospora crassa controls several major events in both the sexual and asexual phases of the fungal life cycle. This 3235-base-pair DNA segment is not homologous to the comparable genetic region of the A mating type. The unique a and A regions are bordered by nearly identical DNA sequences. ... | Proc Natl Acad Sci U S A 1990 Jul;87(13):4917-21 | 271 | 0 |
Method or reagent | PMID:8514114 | The fission yeast Schizosaccharomyces pombe has no large vacuoles under normal growth conditions, although budding yeasts usually have large central vacuoles. The minimum inhibitory concentration of amphotericin B to S. pombe was 0.5 microgram ml-1; treatment with 0.2 microgram ml-1 for 20 min induced rapid and extensi... | FEMS Microbiol Lett 1993 Apr 15;108(3):265-9 | 149 | 0 |
Phylogeny and evolutionary studies | PMID:12901373 | Phylogenetic analysis of conserved gene families in fission yeast Schizosaccharomyces pombe and brewer's yeast Saccharomyces cerevisiae showed that gene duplications have occurred independently in the same families in each of these two lineages to a far greater extent than expected by chance. These species represent di... | Genome Res 2003 Jun;13(6A):1259-64 | 184 | 0 |
Review or comment | PMID:15603750 | Oxidative stress that generates the reactive oxygen species (ROS) is one of the major causes of DNA damage and mutations. The "DNA damage checkpoint" that arrests cell cycle and repairs damaged DNA has been a focus of recent studies, and the genetically amenable model systems provided by yeasts have been playing a lead... | Mutat Res 2005 Jan 06;569(1-2):13-27 | 243 | 0 |
Curatable | PMID:20081370 | We previously reported genome-wide evidence that the Gcn5 histone acetyltransferase (HAT) is located in the transcribed region of highly expressed genes and that it plays an important role in transcriptional elongation in the fission yeast, Schizosaccharomyces pombe (EMBO Reports 2009; 10:1009-14). Furthermore, the spe... | Cell Cycle 2010 Feb 01;9(3):467-71 | 436 | 1 |
Curatable | PMID:19293830 | Microtubules (MTs) are central to the organisation of the eukaryotic intracellular space and are involved in the control of cell morphology. For these purposes, MT polymerisation dynamics are tightly regulated. Using automated image analysis software, we investigate the spatial dependence of MT dynamics in interphase f... | Mol Syst Biol 2009;5:250 | 227 | 1 |
Curatable | PMID:20016281 | Int6/eIF3e is implicated in tumorigenesis, but its molecular functions remain unclear. We have studied its fission yeast homolog Yin6, reporting that it regulates proteolysis by controlling the assembly/localization of proteasomes, and binds directly to another conserved protein, Moe1. In the present study, we isolated... | Cell Cycle 2010 Jan 01;9(1):147-61 | 327 | 1 |
Curatable | PMID:12376568 | We identified a novel fission yeast gene, ned1(+), with pleiotropic mutations that have a high incidence of chromosome missegregation, aberrantly shaped nuclei, overdeveloped endoplasmic reticulum-like membranes, and increased sensitivity to a microtubule destabilizing agent. Ned1 protein, which was phosphorylated in a... | J Cell Sci 2002 Nov 15;115(Pt 22):4375-85 | 285 | 1 |
Curatable | PMID:10625684 | We have purified the RNA polymerase II holoenzyme from Schizosaccharomyces pombe to near homogeneity. The Mediator complex is considerably smaller than its counterpart in Saccharomyces cerevisiae, containing only nine polypeptides larger than 19 kDa. Five of these Mediator subunits have been identified as the S. pombe ... | J Biol Chem 2000 Jan 14;275(2):1351-6 | 235 | 1 |
Transposon related | PMID:16093710 | Recent studies of the LTR-retrotransposons of Schizosaccharomyces pombe have shed considerable light on their evolution and function. The sequencing of the S. pombe genome allowed analysis of its transposon content. This analysis provides information about the maintenance and loss of transposons in the genome. The resu... | Cytogenet Genome Res 2005;110(1-4):566-74 | 277 | 0 |
Curatable | PMID:21193357 | Mutagenic and cytotoxic apurinic/apyrimidinic (AP) sites are among the most frequent lesions in DNA. Repair of AP sites is initiated by AP endonucleases and most organisms possess two or more of these enzymes. Saccharomyces cerevisiae has AP endonuclease 1 (Apn1) as the major enzymatic activity with AP endonuclease 2 (... | DNA Repair (Amst) 2011 Mar 07;10(3):296-305 | 331 | 1 |
Wrong organism | PMID:12215547 | In the budding yeast Saccharomyces cerevisiae, the Cdc3p, Cdc10p, Cdc11p, Cdc12p, and Sep7p/Shs1p septins assemble early in the cell cycle in a ring that marks the future cytokinetic site. The septins appear to be major structural components of a set of filaments at the mother-bud neck and function as a scaffold for re... | Mol Cell Biol 2002 Oct;22(19):6906-20 | 382 | 0 |
Curatable | PMID:30181366 | The fight against resistance to antifungal drugs requires a better understanding of the underlying cellular mechanisms. In order to gain insight into the mechanisms leading to antifungal drug resistance, we performed a genetic screen on a model organism, Schizosaccharomyces pombe , to identify genes whose overexpressi... | Antimicrob Agents Chemother 2018 11;62(11) | 387 | 1 |
Curatable, low priority | PMID:247991 | The nucleotide sequence of Schizosaccharomyces pombe tRNAPhe was determined to be pG-U-C-G-C-A-A-U-G**-G*-U-G-psi-A-G-D-D-G-G-G-A-G-C-A-psi-G*-A-C-A-G-A-Cm-U-Gm-A-A-Y-A-psi-m5C-U-G-U-U-G-m7G-U*-C-A-U-C-G-G-T-psi-C-G-A-U-C-C-C-G-G-U-U-U-G-U-G-A-C-A-C-C-AOH. This sequence differs from that of S. cerevisiae tRNAPhe in 27 ... | Biochemistry 1978 May 02;17(9):1622-8 | 343 | 1 |
Other | PMID:8535288 | Fission yeast responded to environmental cadmium by producing a family of small Cd-binding peptides, phytochelatins (PCn). A low molecular weight (LMW) complex essentially composed of PC2, and PC3 was produced and then disappeared gradually in 24 hrs after Cd treatment, which served as a transient form for a temporary ... | Biochem Mol Biol Int 1995 Aug;36(6):1169-75 | 225 | 0 |
Curatable | PMID:10397757 | Mammalian Ran-binding protein-1 (RanBP1) and its fission yeast homologue, sbp1p, are cytosolic proteins that interact with the GTP-charged form of Ran GTPase through a conserved Ran-binding domain (RBD). In vitro, this interaction can accelerate the Ran GTPase-activating protein-mediated hydrolysis of GTP on Ran and th... | Mol Biol Cell 1999 Jul;10(7):2175-90 | 337 | 1 |
Curatable | PMID:8062838 | The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are co... | EMBO J 1994 Aug 01;13(15):3638-47 | 197 | 1 |
Wrong organism | PMID:8109174 | The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl-5-aminoimidazole-carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed... | Yeast 1993 Nov;9(11):1251-8 | 181 | 0 |
Curatable | PMID:11086011 | The microtubule cytoskeleton plays a pivotal role in cytoplasmic organization, cell division, and the correct transmission of genetic information. In a screen designed to identify fission yeast genes required for chromosome segregation, we identified a strain that carries a point mutation in the SpRan GTPase. Ran is an... | J Cell Biol 2000 Nov 27;151(5):1101-11 | 273 | 1 |
Wrong organism | PMID:19479821 | During a REMI screen for proteins regulating cytokinesis in Dictyostelium discoideum we isolated a mutant forming multinucleate cells. The gene affected in this mutant encoded a kinase, SepA, which is an ortholog of Cdc7, a serine-threonine kinase essential for septum formation in Schizosaccharomyces pombe. Localizatio... | Cell Motil Cytoskeleton 2009 Nov;66(11):929-39 | 285 | 0 |
Curatable | PMID:12759375 | Using a meiosis-specific subtracted cDNA library of Schizosaccharomyces pombe, we identified meu14+ as a gene whose expression is upregulated during meiosis. Transcription of meu14+ is induced abruptly after the cell enters meiosis. Its transcription is dependent on the meiosis-specific transcription factor Mei4. In me... | J Cell Sci 2003 Jul 01;116(Pt 13):2721-35 | 327 | 1 |
Curatable | PMID:26057668 | After deadenylation and decapping, cytoplasmic mRNA can be digested in two opposite directions: in the 5'-3' direction by Xrn1 or in the 3'-5' direction by the exosome complex. Recently, a novel 3'-5' RNA-decay pathway involving Dis3l2 has been described that differs from degradation by Xrn1 and the exosome. The produc... | Acta Crystallogr D Biol Crystallogr 2015 Jun;71(Pt 6):1284-94 | 308 | 1 |
Method or reagent | PMID:10669867 | We report the complete sequence of two cosmids, SPBC19C7 (34815 bp insert, Accession No. AL023859) and SPBC15D4 (33203 bp insert, Accession No. AL031349), localized on chromosome II of the S. pombe genome. Twelve open reading frames (ORFs) were identified in SPBC19C7 and 16 in SPBC5D4. Two known genes were found on eac... | Yeast 2000 Mar 15;16(4):299-306 | 462 | 0 |
Curatable | PMID:27325172 | Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has bee... | Chromosoma 2017 08;126(4):465-471 | 171 | 1 |
Curatable | PMID:11941510 | Eukaryotic Isa1 is one of several mitochondrial proteins that have been implicated in Fe-S cluster assembly paths in vivo. We report the first biochemical characterization of an eukaryotic member of this family and discuss this in the context of results from in vivo studies and studies of bacterial homologues. Schizosa... | J Biol Inorg Chem 2002 Apr;7(4-5):526-32 | 214 | 1 |
Wrong organism | PMID:9747713 | We have isolated an Arabidopsis BBM II isomerase cDNA from an Arabidopsis cDNA library, by means of functional complementation of the E. coli hisA mutant strain HfrG6. The isolated cDNA encodes a polypeptide of 304 amino acids with a calculated molecular weight of 33,363. Sequence comparison with the HIS6 proteins of y... | Mol Gen Genet 1998 Aug;259(2):216-23 | 280 | 0 |
Other | PMID:9471999 | Using a computerized fluorescence microscope system to observe fluorescently stained cellular structures in vivo, we have examined the dynamics of chromosomes and microtubules during the process of meiosis in the fission yeast Schizosaccharomyces pombe. Fission yeast meiotic prophase is characterized by a distinctive t... | J Cell Sci 1998 Mar;111 ( Pt 6):701-12 | 395 | 0 |
Curatable | PMID:24876389 | The Tsc1-Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino acid uptake and fou... | Biol Open 2014 May 29;3(6):542-52 | 314 | 1 |
Curatable | PMID:16415366 | In the fission yeast Schizosaccharomyces pombe the septation initiation network (SIN) is required for stabilization of the actomyosin ring in late mitosis as well as for ring constriction and septum deposition. In a genetic screen for suppressors of the SIN mutant sid2-250, we isolated a mutation, ace2-35, in the trans... | Genetics 2006 Apr;172(4):2101-12 | 355 | 1 |
Curatable | PMID:29695507 | Mmi1 is an essential RNA-binding protein in the fission yeast Schizosaccharomyces pombe that eliminates meiotic transcripts during normal vegetative growth. Mmi1 contains a YTH domain that binds specific RNA sequences, targeting mRNAs for degradation. The YTH domain of Mmi1 uses a noncanonical RNA-binding surface tha... | J Biol Chem 2018 06 15;293(24):9210-9222 | 251 | 1 |
Phylogeny and evolutionary studies | PMID:18577206 | To date very few incidences of interdomain gene transfer into fungi have been identified. Here, we used the emerging genome sequences of Candida albicans WO-1, Candida tropicalis, Candida parapsilosis, Clavispora lusitaniae, Pichia guilliermondii, and Lodderomyces elongisporus to identify recent interdomain HGT events.... | BMC Evol Biol 2008 Jun 24;8:181 | 405 | 0 |
Review or comment | PMID:19269359 | The initiation of DNA replication is a complex, multistep process with important implications for genomic stability. In this issue, Wu and Nurse (2009) find that initiation factors are differentially recruited to replication origins. They uncover evidence suggesting that the efficiency of this recruitment may determine... | Cell 2009 Mar 06;136(5):812-4 | 71 | 0 |
Curatable | PMID:11266451 | We have purified a complex from Saccharomyces cerevisiae containing the spindle components Ndc80p, Nuf2p, Spc25p, and Spc24p. Temperature-sensitive mutants in NDC80, SPC25, and SPC24 show defects in chromosome segregation. In spc24-1 cells, green fluorescence protein (GFP)-labeled centromeres fail to split during spind... | J Cell Biol 2001 Jan 22;152(2):349-60 | 269 | 1 |
Curatable | PMID:18854158 | Mre11 forms the core of the multifunctional Mre11-Rad50-Nbs1 (MRN) complex that detects DNA double-strand breaks (DSBs), activates the ATM checkpoint kinase, and initiates homologous recombination (HR) repair of DSBs. To define the roles of Mre11 in both DNA bridging and nucleolytic processing during initiation of DSB ... | Cell 2008 Oct 03;135(1):97-109 | 276 | 1 |
Curatable | PMID:27756188 | The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, meiosis, and stress responses in Schizosaccharomyces pombe. We demonstrated that Pka1 is responsible for calcium tolerance. Loss of functional components of the PKA pathway such as Git3, Gpa2, Cyr1, and Pka1 yields a CaCl 2 -sensitive pheno... | Biosci Biotechnol Biochem 2017 Feb;81(2):231-241 | 250 | 1 |
Wrong organism | PMID:8917314 | In response to phosphorus limitation, the fungus Neurospora crassa synthesizes a number of enzymes that function to bring more phosphate into the cell. The NUC-2 protein appears to sense the availability of phosphate and transmits the signal downstream to the regulatory pathway. The nuc-2+ gene has been cloned by its a... | Mol Gen Genet 1996 Oct 28;252(6):709-16 | 304 | 0 |
Wrong organism | PMID:2555782 | Nucleotide sequencing of the vaccinia virus SalI F DNA fragment identified an open reading frame of 552 amino acids encoding a protein of 63.3 kDa. The deduced amino acid sequence shares 30% identity with S. pombe and S. cerevisiae DNA ligases, with homology strongest near the carboxy terminus and around the lysine res... | Nucleic Acids Res 1989 Nov 25;17(22):9051-62 | 198 | 0 |
Review or comment | PMID:17219024 | The aim of this review is threefold. First, we want to report on recent observations on the role of telomeres in the alignment of homolog and non-homologues in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe and the relationship of early telomere clustering to later recombination events. Second, we co... | Cell Mol Life Sci 2007 Jan;64(2):125-30 | 132 | 0 |
Curatable | PMID:11416129 | In Schizosaccharomyces pombe, the catalytic subunit of DNA polymerase epsilon (Pol epsilon) is encoded by cdc20(+) and is essential for chromosomal DNA replication. Here we demonstrate that the N-terminal half of Pol epsilon that includes the highly conserved polymerase and exonuclease domains is dispensable for cell v... | Mol Cell Biol 2001 Jul;21(14):4495-504 | 335 | 1 |
Curatable | PMID:7889932 | The structure of a truncated variant of casein kinase-1 from Schizosaccharomyces pombe, has been determined in complex with MgATP at 2.0 A resolution. The model resembles the 'closed', ATP-bound conformations of the cyclin-dependent kinase 2 and the cAMP-dependent protein kinase, with clear differences in the structure... | EMBO J 1995 Mar 01;14(5):1015-23 | 130 | 1 |
Curatable | PMID:27345571 | It is well established that eukaryotic genomes are pervasively transcribed producing cryptic unstable transcripts (CUTs). However, the mechanisms regulating pervasive transcription are not well understood. Here, we report that the fission yeast CENP-B homolog Abp1 plays an important role in preventing pervasive transcr... | Biochim Biophys Acta 2016 10;1859(10):1314-21 | 236 | 1 |
Curatable | PMID:16303567 | Meiosis is a specialized form of cell division by which sexually reproducing diploid organisms generate haploid gametes. During a long prophase, telomeres cluster into the bouquet configuration to aid chromosome pairing, and DNA replication is followed by high levels of recombination between homologous chromosomes (hom... | Curr Biol 2005 Nov 22;15(22):2056-62 | 259 | 1 |
Wrong organism | PMID:29117528 | Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has... | Biophys J 2017 Nov 07;113(9):2055-2067 | 295 | 0 |
Wrong organism | PMID:31796523 | UDP- glucose: glycoprotein glucosyltransferase (UGGT) is a protein that operates as the gatekeeper for the endoplasmic reticulum (ER) quality control mechanism of glycoprotein folding. It is known that vertebrates and Caenorhabditis genomes harbor two uggt gene copies that exhibit differences in their properties.Ba... | G3 (Bethesda) 2020 02 06;10(2):755-768 | 437 | 0 |
Review or comment | PMID:26104357 | Cells of the highly diverged Schizosaccharomyces (S.) pombe and S. japonicus fission yeasts exist in one of two sex/mating types, called P (for plus) or M (for minus), specified by which allele, M or P, resides at mat1. The fission yeasts have evolved an elegant mechanism for switching P or M information at mat1 by a p... | Microbiol Spectr 2014 Oct;2(5) | 322 | 0 |
Curatable | PMID:12932737 | Ars3002 is an efficient single-copy replication origin in the fission yeast, Schizosaccharomyces pombe. In a previous study, we tested the effects of consecutive approximately 50-bp deletions throughout ars3002 on the replication efficiency of those origins in S. pombe. Here we report the results of our use of the same... | Plasmid 2003 Sep;50(2):113-9 | 185 | 1 |
Curatable | PMID:17435009 | Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose expression is abruptly induced at the horsetail phase and which remains expressed until sporulation is finished. Fluorescen... | Eukaryot Cell 2007 Jun;6(6):971-83 | 283 | 1 |
Wrong organism | PMID:1608450 | In budding yeast many genes are expressed under cell-cycle control in late G1. These include a large group of DNA synthesis genes, the HO gene involved in mating-type switching, CTS1 (chitinase) and also CLN1 and CLN2 (ref. 4) encoding G1 cyclins. Two factors, encoded by the SWI4 and SWI6 genes, are required for HO (re... | Nature 1992 Jun 11;357(6378):505-8 | 359 | 0 |
Wrong organism | PMID:16496002 | Many high-throughput loss-of-function analyses of the eukaryotic cell cycle have relied on the unicellular yeast species Saccharomyces cerevisiae and Schizosaccharomyces pombe. In multicellular organisms, however, additional control mechanisms regulate the cell cycle to specify the size of the organism and its constitu... | Nature 2006 Feb 23;439(7079):1009-13 | 320 | 0 |
Wrong organism | PMID:12077342 | A growing body of evidence supports the coordination of pre-mRNA processing and transcriptional regulation. We demonstrate here that mammalian PRP4 kinase (PRP4K) is associated with complexes involved in both of these processes. PRP4K is implicated in pre-mRNA splicing as the homologue of the Schizosaccharomyces pombe ... | Mol Cell Biol 2002 Jul;22(14):5141-56 | 376 | 0 |
Wrong organism | PMID:1386897 | Entry of yeast cells into the mitotic cell cycle (Start) involves a form of the CDC28 kinase that associates with G1-specific cyclins encoded by CLN1 and CLN2 (ref. 1). The onset of Start may be triggered by the activation of CLN1 and CLN2 transcription in late G1 (ref. 2). SWI4 and SWI6 are components of a factor (SBF... | Nature 1992 Aug 13;358(6387):593-7 | 304 | 0 |
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